Gram Stain Explained | Principle, Procedure, Interpretation & Clinical Utility
23:38
YouTubePrashanth P
Gram Stain Explained | Principle, Procedure, Interpretation & Clinical Utility
Gram staining is one of the most fundamental and rapid diagnostic tools in clinical microbiology. This video provides a complete, exam-oriented and clinically relevant overview of Gram stain, covering both theory and practical application. In this video, you will learn: Principle of Gram staining Components and reagents used Step-by-step Gram ...
2 days ago
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Gram staining is a differential staining technique used in microbiology to classify bacteria into Gram-positive and Gram-negative based on the composition of their cell wall. Prepare smear → thin film of bacteria on slide Air dry Heat fix by passing slide through flame Add Crystal Violet → 1 minute → rinse Add Gram’s Iodine → 1 minute → rinse Decolorize with alcohol/acetone → few seconds Rinse immediately Add Safranin (counter stain) → 30–60 sec → rinse Air dry and observe under oil immersion (1
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