The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...

Optimizing PCR assays for assay development (AD) workflows typically involves a range of interdependent factors. These include thermocycling conditions, enzyme levels, buffer chemistry, and ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...