Small hairpin RNA (shRNA) is a powerful tool for inhibiting gene expression. One limitation has been that this technique has been used primarily to target a single gene. This protocol expands upon ...

In vivo protein expression includes cloning a gene of interest with the essential control elements into an expression vector, and then transforming the vector into an easily grown organism or cell ...

Proteins are the fundamental building blocks of life, and they are the essential products of molecular biology’s core dogma. Proteins play a variety of roles in the body, including catalyzing ...

Escherichia coli is widely considered the most cost-effective bacterial system for recombinant protein expression. Consequently, it is broadly used. Nonetheless, it has its limitations as an ...

Recombinant protein expression is an essential pillar of biomedical research. Efforts from enzyme discovery to protein structure-function study to development of drugs and biological therapeutics all ...